PROSPERO (PRediction of Outcome using Sequence & Protein Experimental Results Online) estimates your chances of getting diffracting crystals from purified protein, based on both results of experimental protein characterization and analysis of sequence. To use the predictor,

Summary of the method used in PROSPERO. [Download PDF]

The original predictor is based on experimental results, sequences and outcomes from protozoan structural genomics projects, SGPP and MSGPP. See "Prediction of protein crystallization outcome using a hybrid method" for details.

PROSPERO is interested in collecting experimental results, sequences and outcomes from other structural genomics and structural biology labs to develop predictive models for specific sets of proteins and to improve the predictor. Please contact Dr. Ethan Merritt if you would like to collaborate.

Please cite the HyGX1 prediction algorithm as: Zucker, F.H., et al. "Prediction of protein crystallization outcome using a hybrid method" J. Struct. Biol. 151:1 (2010) 64-73, 10.1016/j.jsb.2010.03.016. Access from JSB or download the pdf. Cite this web server as: Zucker, F.H., Kim, H.Y. and Merritt, E.A. "PROSPERO: online prediction of crystallographic success from experimental results and sequence" J. Appl. Cryst. 45:3 598-602, 10.1107/S002188981201775X. Access from JAC or download the pdf.

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