| 30 Mar 2010 | JobID: TLSMD7784_zxWfiZTD | TLSMD Version 1.4.1 |
| Title | STRUCTURAL BASIS FOR GROEL-ASSISTED PROTEIN FOLDING FROMTHE CRYSTAL STRUCTURE OF (GROEL-KMGATP)14 AT 2.0 ARESOLUTION |
| Heading Summary | 1KP8:CHAPERONE:30-DEC-01 |
| Experimental Method | X-RAY DIFFRACTION |
| Temperature Factors | Isotropic |
| Minimum TLS Segment Length | 4 Residues |
| Atoms Analyzed | ALL |
For each protein chain, this analysis show the optimal division into 1 TLS group, 2 TLS groups, 3 TLS groups, etc, up to 20 groups. It goes on to analyze the implied rigid body translational and rotational motion of each group, as well as its quality of fit to the refined atomic displacement parameters (B-factors). If you have multiple chains in your structure, they are treated independently. |  |
When multiple chains are present in your input structure, a side-by-side sequence alignment is generated to show how the TLS group selection for one chain aligns with the selection for the other chains. This analysis is only meaningful when there are multiple chains of the same sequence in the asymmetric unit.
1KP8: Multi-Chain Alignment Analysis of TLS Groups
The CCP4 refinement program Refmac5 and the PHENIX program PHENIX.refine both implement a refinement mode in which individual isotropic temperature factors are supplemented by refinement of one or more TLS groups. TLSMD will help you choose how many TLS groups to subdivide your protein chains into, and it will generate a input files for you to use in further refinement using Refmac5 or PHENIX.
1KP8: Generate input files for multigroup TLS Refinement
| 30 Mar 2010 | JobID: TLSMD7784_zxWfiZTD | TLSMD Version 1.4.1 Released 11 February 2010 |