Legend for each sample's set of figures

Each sample has three rows (fit, transitions, derivatives) and at least as many columns as transitions in the final model. For all three rows: observed fluorescence or derivative, black '+'; smoothed observations, black curve; fitted model, red curve.
Complete legends:
Differential Scanning Fluorimetry Curve Iterative Fitting. Final fitted model, column with pale green background above top row and thin green outline; intermediate steps in iteration towards final model, if any, to the left with grey background; possible additional transition tested and rejected (e.g. for not significantly improving the model), to the right with pink background.
Overall model, top row: Fluorescence Intensity (arbitrary units) vs Temperature (°C). Observed fluorescence, black '+'; smoothed observations, thick black curve; fitted model, thin red curve; residual (smoothed observations - fit), thin green curve; background from model, thin blue curve. Transition midpoint temperature (T_m) and transition intensity range from I_min to I_max, vertical thick blue bar(s); transition temperature range (full width at half maximum slope, FWHM) and intensity at transition midpoint (I_Tm), horizontal thick green bar(s).
Individual transitions, middle row: Fluorescence with background (bg) subtracted vs Temperature. Observed fluorescence minus bg , black '+'; smoothed observations minus bg, black curve; fitted model minus bg, red curve. Individual transition models with I_min subtracted, thick colored curves. Individual transition curves and vertical blue bars at T_m go from zero to ΔI = I_max - I_max so that it is easier to compare intensity changes among transitions. Green bars show temperature range of transition as in top row, at ΔI /2.
Derivatives, bottom row: Derivative of fluorescence intensity (dI/dT) vs Temperature. Observed derivative, black '+'; smoothed derivative, black curve; fitted model, red curve. Derivatives of individual transitions, curves colored as in middle row but using thin lines for clarity. For exponentially decaying backgrounds: derivative of model background, thin dark blue curve; smoothed observed derivative with that background derivative subtracted, thin green curve. Individual transition peaks should fit this curve. (For constant backgrounds, these curves overlap the X axis and the black line, respectively). T_m and maximum slope from the model, vertical blue bars; temperature range at half-maximum slope (spanning peaks at half height), horizontal green bars.
Curve Fitting: See summary

Sample 1: A1 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 1 Fitted	FINAL FIT    intensity plateaus at 52.7 C	Not used: R_abs change below threshhold
Sample 1 Transitions
Sample 1 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 2: A2 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 2 Fitted	not used	FINAL FIT	Not used: no positive slope left
Sample 2 Transitions
Sample 2 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 3: A3 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 3 Fitted	not used    intensity plateaus at 62.5 C	FINAL FIT    intensity plateaus at 62.5 C ? std. dev. above 2 C	Not used: Estimated intensity change below threshhold
Sample 3 Transitions
Sample 3 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 4: A4 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 4 Fitted	FINAL FIT    sudden intensity jump at 55.1 C	Not used: No transition within temperature range
Sample 4 Transitions
Sample 4 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 5: A5 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 5 Fitted	FINAL FIT    sudden intensity jump at 53.9 C	Not used: Estimated intensity change below threshhold
Sample 5 Transitions
Sample 5 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 6: A6 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 6 Fitted	not used    intensity plateaus at 62.1 C	FINAL FIT    intensity plateaus at 62.1 C ? ! std. dev. above 10 C	Not used: R_abs change below threshhold
Sample 6 Transitions
Sample 6 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 7: A7 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 7 Fitted	FINAL FIT    intensity plateaus at 53.7 C	Not used: Estimated intensity change below threshhold NO CURVE FITTING ATTEMPTED
Sample 7 Transitions		NO CURVE FITTING ATTEMPTED
Sample 7 Derivatives		NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 8: A8 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 8 Fitted	FINAL FIT	Not used: No transition within temperature range
Sample 8 Transitions
Sample 8 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 9: A9 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 9 Fitted	FINAL FIT	Not used: Estimated intensity change below threshhold
Sample 9 Transitions
Sample 9 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 10: A10 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 10 Fitted	not used	FINAL FIT	Not used: no positive slope left
Sample 10 Transitions
Sample 10 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 11: A11 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 11 Fitted	not used    intensity plateaus at 61.3 C	FINAL FIT    intensity plateaus at 61.3 C	Not used: Tms too close together
Sample 11 Transitions
Sample 11 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 12: A12 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 12 Fitted	not used	FINAL FIT	Not used: no positive slope left
Sample 12 Transitions
Sample 12 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 13: B1 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 13 Fitted	FINAL FIT	Not used: Estimated intensity change below threshhold NO CURVE FITTING ATTEMPTED
Sample 13 Transitions		NO CURVE FITTING ATTEMPTED
Sample 13 Derivatives		NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 14: B2 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 14 Fitted	FINAL FIT    sudden intensity jump at 53.7 C	Not used: Estimated intensity change below threshhold NO CURVE FITTING ATTEMPTED
Sample 14 Transitions		NO CURVE FITTING ATTEMPTED
Sample 14 Derivatives		NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 15: B3 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 15 Fitted	not used    sudden intensity jump at 53.7 C	FINAL FIT    sudden intensity jump at 53.7 C	Not used: Estimated intensity change below threshhold NO CURVE FITTING ATTEMPTED
Sample 15 Transitions			NO CURVE FITTING ATTEMPTED
Sample 15 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 16: B4 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 16 Fitted	FINAL FIT	Not used: Intensity change of transition below threshhold
Sample 16 Transitions
Sample 16 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 17: B5 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 17 Fitted	FINAL FIT    sudden intensity jump at 58.9 C	Not used: No transition within temperature range NO CURVE FITTING ATTEMPTED
Sample 17 Transitions		NO CURVE FITTING ATTEMPTED
Sample 17 Derivatives		NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 18: B6 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 18 Fitted	FINAL FIT    ? major transition not near max. slope	Not used: No transition within temperature range
Sample 18 Transitions
Sample 18 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 19: B7 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 19 Fitted	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 19 Transitions		NO CURVE FITTING ATTEMPTED
Sample 19 Derivatives		NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 20: B8 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 20 Fitted	FINAL FIT    ? major transition not near max. slope	Not used: Intensity change of transition below threshhold
Sample 20 Transitions
Sample 20 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 21: B9 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 21 Fitted	Not used: No positive slope
Sample 21 Transitions
Sample 21 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv
Sample 22: B10 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 22 Fitted	FINAL FIT    ? major transition not near max. slope	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 22 Transitions		NO CURVE FITTING ATTEMPTED
Sample 22 Derivatives		NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 23: B11 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 23 Fitted	Not used: No positive slope
Sample 23 Transitions
Sample 23 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv
Sample 24: B12 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 24 Fitted	FINAL FIT	Not used: Estimated intensity change below threshhold NO CURVE FITTING ATTEMPTED
Sample 24 Transitions		NO CURVE FITTING ATTEMPTED
Sample 24 Derivatives		NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 25: C1 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 25 Fitted	FINAL FIT	Not used: Estimated intensity change below threshhold
Sample 25 Transitions
Sample 25 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 26: C2 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 26 Fitted	FINAL FIT	Not used: no positive slope left
Sample 26 Transitions
Sample 26 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 27: C3 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 27 Fitted	FINAL FIT	Not used: no positive slope left
Sample 27 Transitions
Sample 27 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 28: C4 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 28 Fitted	FINAL FIT	Not used: no positive slope left
Sample 28 Transitions
Sample 28 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 29: C5 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 29 Fitted	FINAL FIT	Not used: Estimated intensity change below threshhold
Sample 29 Transitions
Sample 29 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 30: C6 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 30 Fitted	FINAL FIT	Not used: no positive slope left
Sample 30 Transitions
Sample 30 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 31: C7 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 31 Fitted	FINAL FIT	Not used: Estimated intensity change below threshhold
Sample 31 Transitions
Sample 31 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 32: C8 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 32 Fitted	FINAL FIT	Not used: Estimated intensity change below threshhold
Sample 32 Transitions
Sample 32 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 33: C9 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 33 Fitted	FINAL FIT	Not used: no positive slope left
Sample 33 Transitions
Sample 33 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 34: C10 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 34 Fitted	FINAL FIT	Not used: no positive slope left
Sample 34 Transitions
Sample 34 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 35: C11 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 35 Fitted	FINAL FIT	Not used: no positive slope left
Sample 35 Transitions
Sample 35 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 36: C12 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 36 Fitted	FINAL FIT	Not used: no positive slope left
Sample 36 Transitions
Sample 36 Derivatives
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 37: D1 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 37 Fitted	not used	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 37 Transitions				NO CURVE FITTING ATTEMPTED
Sample 37 Derivatives				NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 38: D2 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 38 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 38 Transitions			NO CURVE FITTING ATTEMPTED
Sample 38 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 39: D3 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 39 Fitted	not used	FINAL FIT	Not used: Estimated intensity change below threshhold NO CURVE FITTING ATTEMPTED
Sample 39 Transitions			NO CURVE FITTING ATTEMPTED
Sample 39 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 40: D4 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 40 Fitted	not used	FINAL FIT	Not used: Estimated intensity change below threshhold NO CURVE FITTING ATTEMPTED
Sample 40 Transitions			NO CURVE FITTING ATTEMPTED
Sample 40 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 41: D5 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 41 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 41 Transitions			NO CURVE FITTING ATTEMPTED
Sample 41 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 42: D6 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 42 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 42 Transitions			NO CURVE FITTING ATTEMPTED
Sample 42 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 43: D7 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 43 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 43 Transitions			NO CURVE FITTING ATTEMPTED
Sample 43 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 44: D8 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 44 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 44 Transitions			NO CURVE FITTING ATTEMPTED
Sample 44 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 45: D9 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 45 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 45 Transitions			NO CURVE FITTING ATTEMPTED
Sample 45 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 46: D10 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 46 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 46 Transitions			NO CURVE FITTING ATTEMPTED
Sample 46 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 47: D11 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 47 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 47 Transitions			NO CURVE FITTING ATTEMPTED
Sample 47 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data
Sample 48: D12 (summary)
NEXT	1 Transition	2 Transitions	3	4	5
Sample 48 Fitted	not used	FINAL FIT	Not used: no positive slope left NO CURVE FITTING ATTEMPTED
Sample 48 Transitions			NO CURVE FITTING ATTEMPTED
Sample 48 Derivatives			NO CURVE FITTING ATTEMPTED
	PREVIOUS       Legend & Index       Results Table ../fit.tmc.csv       GnuPlot commands to fit and plot data